The total flavonoid content in the samples demonstrated that these are the major phenolic compounds. The highest content of phenolic compounds was found in the crude extract, followed by the ethyl acetate fraction. Photoprotective gel formulations were developed with the extract of H. An HPLC-ESI-IT profile of flavonoids was developed. Total phenolic compounds, total flavonoids, sun protection factor (SPF) and antioxidant activity of the crude extract were determined. In this context, this work aimed to perform high-performance liquid chromatography-mass spectrometry-electrospray ionization-ion trap (HPLC-MS-ESI-IT) to characterize the flavonoids from Hymenaea martiana bark extract and develop a cosmetic formulation with photoprotective action. Hymenaea martiana Hayne has flavonoids related to its pharmacological activities, but a flavonoid profile of this species has not been reported yet. The development of photoprotective products has been highly focused on natural ingredients, and exposure to ultraviolet (UV) radiation is also related to the production of reactive oxygen species (ROS), which are extremely damaging to tissues.
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